RNA sequencing to analyze trasncriptional profiles and screen potential regulators in confined migration

To identify potential regulators involved in confined cell migration, we performed transwell migration assay with 3 μm pore size to simulate native physical confined microenvironment. Transmigrated cells were collected for RNA sequencing to analyze transcriptional profile changes in comparison to parental cells. Here are two types of samples, namely control and 3 μm_confine. The control group represents parental MEF cells. The 3 μm_confine group represents MEF cells transmigrated through transwell with 3 μm pore size.