RNA-seq of human cancer cell line OVCA420* following TIGAR knockdown with shRNA

To identify differentially expressed genes following TIGAR knockdown, OVCA420* was transduced with TIGAR-targeting or non-targeting (control) shRNAs. 54 hours after the transduction, RNA was extracted and Illumina TruSeq RNA Sample Preparation Kit v2 was used to prepare the libraries. Each group contains 3 replicates. NextSeq 500 was used for sequencing.