Molecular characterization of antimicrobial resistant Pseudomonas aeruginosa isolates in tertiary care hospital, Kathmandu

Molecular characterizing Pseudomonas aeruginosa isolates that are resistant to antibiotics in a tertiary care hospital, like one in Kathmandu, means figuring out the exact genetic factors and mechanisms that cause the resistance. The study collects samples, tests them for antimicrobial susceptibility (AST), and then uses Clinical and Laboratory Standards Institute (CLSI) guidelines to decide whether the isolates are susceptible, intermediate, or resistant.Molecular characterization involves detecting resistance genes using PCR, gene sequencing, and plasmid analysis. Genotyping involves pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and whole genome sequencing (WGS). Data analysis includes assessing the prevalence of resistance to different antibiotics, examining the association with clinical outcomes, and identifying epidemiological trends in the spread of resistant strains within the hospital.Conclusions and recommendations include infection control measures, antibiotic stewardship, and further research. The findings could help in understanding the molecular mechanisms behind antimicrobial resistance in Pseudomonas aeruginosa in a specific hospital setting, aiding in the development of targeted interventions to control the spread of resistant strains. Further research could investigate resistance mechanisms in greater detail or alternative treatment options.