Identifying miR-139 targets in motor neurons

We sought to investigate changes in expression levels of RNAs in response to miR-139 overexpression in human iPSC-derived motor neurons Motor neurons were differentiated from FUS H517Q iPSCs and transduced with AAVs expressing miR-139 or controls hairpins. RNA was harvested at day 34. We performed gene expression profiling analysis using data obtained from bulk RNA-seq. Comparative gene expression profiling using RNA-seq analysis of miR-139 overexpression in FUS H517Q motor neurons.