RNA-sequencing comparing the transcriptome of adventitial LepR+ fibroblasts versus their interstitial LepR- counterparts. [mouse RNA-Seq]

In this study, we investigated the role of LepR-Cre+ cardiac fibroblasts (CFs). Our results reveal a specific gene expression profile of LepR-Cre+ CFs cells compared with LepR-Cre- CFs in both the postnatal period (P7) and adulthood (P56). Our findings support a model in which adventitial fibroblasts, being characterized by upregulation of genes involved in critical functions, including hedgehog signaling, blood pressure regulation, and interaction with the immune system, are major contributors in cardiac fibrosis. Overall design: RNA-seq analyses were performed on FACS purified LepR-Cre+ and LepR-Cre- cardiac fibroblasts at P7 and P56.