Pseudomonas kaiseriensis sp nov, isolated from Rainbow Trout. Pseudomonas kaiseriensis sp nov, isolated from Rainbow Trout

Strains P45T P44, P46, P91. P92, P93, P95, P52, P56, P97, V42, V43, V44 and V45 were Gram-stain-negative, short rods, motile by more than one polar flagellum and isolated from Onchorhynchus mykiss, rainbow trouts. The 16S rRNA sequence similarity values grouped them in the Pseudomonas fluorescens phylogenetic group of species. The DNA G+C content ranged from 65.5 to 65.7 mol%. The strains were characterized phenotypically by the API 20NE and Biolog GEN III and VITEK 2 tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and by their fatty acid contents. The absence of the arginine dihydrolase and the hydrolysis of gelatin differentiated these strains from the closest species, Pseudomonas azotifigens. The major fatty acid contents were C16: 0 (35.05 %), summed feature 3 (31. %) and summed feature 8 (13.17 %). Multilocus sequence analysis with four housekeeping gene sequences (rpoD, rpoB, gyrB and 16S rRNA) together with the genome comparisons by ANIb and GGDC values indicated that these strains cluster together in the phylogenetic analysis and their similarity values were lower than the thresholds established for species in the genus Pseudomonas. These data permit us to conclude that strains P45T, P44, P46, P91. P92, P93, P95, P52, P56, P97,V42, V43, V44 and V45 belong to a novel species in the genus Pseudomonas for which the name Pseudomonas kayserensis sp. nov. is proposed. The type strain is P45T.