Microbiome analysis of control, radiation enteritis model, and treatment groups in rat stool samples using 16S rRNA sequencing

This study investigates the gut microbiome composition and diversity in three groups of rats: control, radiation enteritis model, and treatment (TG) groups. Total DNA was extracted from stool samples, PCR-amplified targeting 16S rRNA gene variable regions, and sequenced using Illumina MiSeq or NovaSeq platforms. Downstream bioinformatics analyses included sequence quality control, denoising (DADA2/OTU clustering), taxonomic classification, alpha and beta diversity evaluation, differential species abundance analysis, and functional prediction. The processed data include ASV/OTU tables, taxonomy assignments, and sample metadata. Overall design: Comparative microbiome profiling of three groups (control, model, TG) to assess microbial composition and diversity changes following disease induction and treatment. Each group includes 6–7 biological replicates. 16S rRNA amplicon sequencing was performed, followed by bioinformatics analyses including denoising, OTU clustering, taxonomic annotation, diversity analyses, and functional prediction.