P6 Mouse Sertoli Cell Small RNA seq

This study annotates the NGS reads in a Sertoli cell sncRNA library. In addition to known sncRNAs, we also identified numerous novel sncRNAs expressed by Sertoli cells. Our data suggest that the Sertoli cell sncRNA transcriptome predominantly consists of miRNAs, piRNA-like RNAs, tRNAs and snoRNAs. This study reports the first comprehensive annotation of the Sertoli cell sncRNA transcriptome. Sertoli cells were purified from post-natal day 6 C57Bl/6 mice using the STAPUT unit gravity sedimentation apparatus. Small RNA was isolated from cells and a cDNA library was generated for 454 sequencing. Sequences from FastA files were initially mapped to known sequences and the genome and subsequently annotated upon novel small RNA categorization.本研究对支持细胞（Sertoli cell）的小非编码RNA（small non-coding RNA，简称sncRNA）文库中的下一代测序（Next-Generation Sequencing，简称NGS）读段进行了注释。除已注释的sncRNA外，本研究还鉴定出了支持细胞表达的多种新型sncRNA。本研究数据显示，支持细胞的sncRNA转录组主要由微小RNA（microRNA，简称miRNA）、piRNA样RNA（piRNA-like RNAs）、转运RNA（transfer RNA，简称tRNA）以及核仁小RNA（small nucleolar RNA，简称snoRNA）构成。本研究首次报道了支持细胞sncRNA转录组的全面注释。研究人员采用STAPUT单位重力沉降装置，从出生后第6天的C57Bl/6小鼠体内纯化分离得到支持细胞。从细胞中分离小分子RNA，并构建了用于454测序的cDNA文库。研究人员首先将FASTA格式文件中的测序序列比对至已知序列及基因组，随后基于新型小RNA的分类完成了注释工作。