Additional file 1 of Many kinds of oxidized proteins are present more in the urine of the elderly

Additional file 1. Table S1. Detailed information of individuals. Table S2. The proportion of modifications occurring at each amino acid. Table S3. Differences and statistics of oxidations and AA modifications between different groups. Table S4. ROC performance parameters and Delong test in the discovery cohort. Table S5. Random grouping calculation results in 14 modifications. Table S6. ROC performance parameters and Delong test in validation cohort. Table S7. The urine sample processing methods of the published studies in the literature, and the comparison with this method. Table S8. The urine sample data collection methods of published studies in the literature and compares them with our method. Fig. S1. Oxidative proteins PPI analysis and gene functional analysis using STRING. Fig. S2. Functional profiling of each protein set was conducted using the GO, KEGG and WikiPathways databases. Fig. S3. The differentially expressed proteins overlap between each group. Fig. S4. O-Y-David. BF, MF, CC, and KEGG by David and gene functional analysis by g:Profiler. Fig. S5. O-M-David. BF,MF,CC, and KEGG by David and gene functional analysis by g:Profiler. Fig. S6. PFind parameters setting (Open-search). Fig. S7. a. A illustration of the definition of Species and Sites. As for any modification, “sites” means the quantity of all sites where modifications occur, “species” refers to the type of peptide that is modified. b. Schematic representation of the comparison between the significant modifications via “sites” and “species” aging urinary proteomes. Supplementary site calculate method code: The program codes to extract this information from the “pd.all_result” document.