Data from: Conservation genomics of the threatened Trispot Darter (Etheostoma trisella)

# Etheostoma\_trisella\_population\_genomics\_2023 [https://doi.org/10.5061/dryad.jq2bvq8g6](https://doi.org/10.5061/dryad.jq2bvq8g6) This repository contains the data used to perform population genomic and phylogenetic analyses for *Etheostoma trisella* collected in the United States. The data are the result of DArTSeq: a combination of DArT complexity reduction with sequencing on the Next Generation Sequencing platforms. It represents combined marker discovery and genotyping for SNPs. Approximately 50,000 DNA fragments from DArT representations were assayed for polymorphism. We detect three distinct management units (i.e., genetic groups) which reflect isolated geographic localities (i.e., Little Canoe Creek, Ballplay Creek, and a system including the Conasauga and Coosawattee rivers). We also detected a recent bottleneck event in the Ballplay Creek population as well as a low effective population size. Tests of isolation by distance further suggest that populations are structured by riverine isolation rather than geographic distance. ## Description of the data and file structure The SNP report file in .csv format includes data for 93 *Etheostoma trisella* specimens. The file includes columns for AlleleID, CloneID, AlleleSequence, TrimmedSequence, SNP, SnpPosition, CallRate, OneRatioRef, OneRatioSnp, FreqHomRe, FreqHomSnp, FreqHets, PICRef, PICSnp, AvgPIC, AvgCountRef, AvgCountSnp, RepAvg. Binary states for loci are also indicated for each specimen. Specimens are named with a unique number identifier followed by collection locality. ## Sharing/Access information Not applicable. ## Code/Software Not applicable.