Optimized Bone-In-Culture Array to study prostate cancer bone metastasis

Based on RNA-seq, we performed transcriptomic profiling to examine the similarities or differences between BICA (Bone-in-culture array) and IVBL (in vivo bone lesion). Transcriptomic analysis and Principle component analysis indicated that the transcriptomic profiles of cancer cells in BICA are more closely mimicking IVBL, as compared to cancer cells in 2D and in orthotopic tumors. These results provide transcriptome-wide evidence supporting BICA as a platform to mimic microenvironment of prostate cancer bone metastasis.