Enhanced sensitivity of isogenic diploid and haploid deletion strains to doxorubicin, hydroxyurea (HU) or methyl methanesulfonate (MMS) when compared to repair competent (WT) parental strain.

*These deletion strains were detected in the haploid DOX screen [38].
1Yeast deletions identified in the diploid deletion DOX screen were cross sensitive to ionizing radiation (see [40], [39]) and Table 1. Bold indicates deletion strains that showed diploid-specific enhanced sensitivity to DOX.
2Relative sensitivity of the diploid (2n) versus haploid (1n) deletion strains to DOX was determined at a concentration of 50 ug/ml. Cells were grown in liquid YPD for two days and serial 5 fold dilutions made in sterile water. Two ul aliquots were then spotted to YPD and the DOX plates and allowed to grow for 3 days. >SSS denotes an enhanced sensitivity for a given deletion mutant that was greater than 125 fold over that observed for the isogenic WT of the same ploidy; SSS denotes a 125 fold enhanced sensitivity of the mutant when compared to WT; SS denotes a 25 fold enhanced sensitivity of the mutant when compared to WT; S denotes a five fold enhanced sensitivity of the mutant when compared to WT; “ - “ denotes no enhanced sensitivity of the mutant when compared to WT.
3Relative sensitivity to hydroxyurea (HU) was determined at 200 uM.
4Relative sensitivity to methyl methanesulfonate (MMS) was determined at 2 uM.