Transcriptome Analysis of HMGB1 knock-out iSLK BAC16 cell (2)

Purpose: RNA sequencing (RNA-seq) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. In addition, we have already registered the transcriptome analysis results of HMGB1 KO iSLK BAC16 cell in GSE157275, and this registration is to reproduce it. HMGB1 WT or KO iSLK BAC16 cells transcriptome were analyzed by RNA seq. cells were or were not induced KSHV lytic replication with doxycycline and sodium butyrate.