Massively parallel splicing reporter assay on designed sequence libraries. Massively parallel splicing reporter assay on designed sequence libraries

We designed 4 oligonucleotide libraries containing either a retained intron, a cassette exon, tandem 5' or tandem 3' splice sites, cloned them into dedicated reporter constructs, transfected and integrated these constructs in the genome of K562 cells, and performed targeted RNA sequencing to determine RNA splicing ratios and a FACSseq approach to determine protein isoform ratios. Overall design: Massively parallel reporter assay for alternative splicing