Regulation of Host Gene Expression by Gastrointestinal Tract Microbiota

Difference in gut microbiome is linked with health, disease and eventually host fitness, however, the molecular mechanisms by which this variation affects the host fitness are not well characterized. Here, we modified the fish gut microbiota by using antibiotic and probiotic to address the effect of host microbiome on gene expression pattern by using transcriptome. Overall design: Chinook salmon (Oncorhynchus tshawytscha) gene expression was evaluated using transcriptome sequencing (RNA-Seq) on hindgut mucosa samples from individuals reared in the presence of antibiotics and probiotics and healthy controls to determine differentially expressed (DE) host genes.