Two photon calcium imaging in the CA1 region of the hippocampus in neonatal mice.
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We performed in vivo 2-photon calcium imaging in the CA1 region of the hippocampus in awake mouse pups aged between 5 and 12 days postnatal. We used GCaMP6s calcium indicator in WT mice or GCaMP6s and flex-tdTomato in GadCre mice to record calcium dynamics from both pyramidal cells and interneurons. The imaging data set was acquired at 8 Hz, in field of view of 400x400 µm. Simultaneously with imaging, we record the spontaneous motor behavior of the mouse pups.
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DANDI Archive创建时间:
2022-01-25



