High-resolution mapping of budding yeast Nab3, Nrd1, and RNA polymerase II by CRAC in wild-type and rrp6∆ strains.

We obtained Nab3, Nrd1, and RNA polymerase II occupancy profiles across the genome of S.cerevisiae in wild-type and rrp6∆ strains. This allowed us to determine the impact of defective nuclear exosome on NNS-dependent transcription termination.我们获取了野生型及rrp6Δ菌株中酿酒酵母（S. cerevisiae）全基因组范围内Nab3、Nrd1以及RNA聚合酶II（RNA polymerase II）的占据谱。借此我们得以明确功能缺陷的核外切体（nuclear exosome）对NNS依赖型转录终止的影响。