Expression data for the molecular signature of TF1a acute myeloid leukaemia cell line

TF1a AML cell line was selected for in vitro modelling of dormancy in AML. TF1-a were subjected to AML-niche-mimicking in vitro conditioning by culture with TGFB1 and the mTOR inhibitor rapamycin. Also TF1a cells were in vitro cultured with prolonged sublethal doses of Etoposide. The molecular signature of untreated, dormancy induced and genotoxicity-surviving TF1a cells were investigated using global human genome GEP to identify drivers of dormancy in different conditioning scenarios in comparison to untreated counterparts. TF1a cells were cultured for 3 days with TGFB1 or rapamycin (mTOR inhibitor that mimickes scarcity of neutrients) or combination of the tow dormancy inducer. Also TF1a cells were cultured with IC20 doses of Etoposide for 6 days. At the end of the treatmnt incubation duration, TF1a were harvested and total RNA were extracted for the GEP analysis.