Loss of Cfl1 dose-dependently causes epiboly defect.

Note: Embryos at 1-cell stage were injected without (sham-injected with 2.3 µL injection buffer) or with different dosages of cfl1 tMO1 or tMO2 and examined at 10 hpf. Some embryos died (usually <10%) at 10 hpf and were not counted in these experiments. Embryos did not reached 100% epiboly were considered to have epiboly defect. These experiments were repeated 4 times. Data are presented as means ± standard deviation. Experimental values are compared within groups, subjected to ANOVA and the mean separation was done by Duncan's Multiple Range Test (DMRT). Values with different lettering in their superscripts are significantly (p<0.05) from each other.