Postnatal SETD1B is essential for learning and the regulation of neuronal plasticity genes

We have analyzed changes in histone 3 lysine 4 methylation (H3K4me3 and H3K4me1), histone acetylation (H3K9ac and H3K27ac) and gene expression resulting from Setd1b knockdown in adult hippocampal CA neurons in mice. We implemented Cre/loxP-mediated conditional knockout strategy in order to knockdown this histone methyltransferase in excitatory forebrain neurons in adult mice. Overall design: Examination of H3K4me3, H3K4me1, H3K9ac and H3K27ac levels, and gene expression in hippocampal CA neurons of adult Setd1b cKO mice.