RALY selectively regulates the alternative splicing of transcription factors and the expression of immune/ inflammatory response genes in HeLa cells

We applied a HeLa cell model with enforced expression of RALY and studied the effect of RALY on cancer cell proliferation and apoptosis. We then obtained RNA-seq transcriptomes from the normal cells and those with RALY overexpression, followed by analysis of the deregulation of alternative splicing and gene expression that were mediated by RALY overexpression. Overall design: We overexpressed RALY in HeLa cells by transfecting Flag with RALY. Negative control was prepared at the same time. Two biological replicates were obtained and prepared for RNA-seq experiments.