Jennifer Bestman, Holly Cline (2011) CIL:36963, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #2 (4 hour) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired at 4 hour intervals over 8 hours, and show morphological plasticity of the dendritic arbor with branch additions and retractions.

These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图像为该图像组中时间序列图像的第二幅（拍摄间隔为4小时）。该图像展示了无色斑马鱼Xenopus laevis神经中枢视束神经元的形态变化，处于第47发育阶段。

该图像系列在8小时内以4小时为间隔进行采集，展现了树突树形态的可塑性变化，包括分支的增加与收缩。

这些细胞在首次图像采集前约24小时通过体内电穿孔转染了编码eYFP的质粒DNA。完整的活体蝌蚪经过麻醉后置于载玻片下，并通过定制构建的双光子显微镜直接穿过其透明皮肤进行成像。