Sample sizes for morphological and physiological measures by rearing treatment and histological stage.

Sample sizes of juvenile male steelhead sampled at Winthrop National Fish Hatchery separated according to rearing treatment (S1, S2), release year (2011–2015), and histological stage (immature, stage 0; maturing, stage 1–4; mature, spermiating). Morphological and physiological measures are represented in S1–S8 Figs with sample sizes corresponding to individual bars. Abbreviations and units of measure are as follows: fork length (mm); body weight (g); gill Na+/K+ ATPase activity (μmol ADP · mg protein-1 · hr-1); pituitary mRNA (follicle stimulating hormone beta-subunit, fshb; luteinizing hormone beta-subunit, lhb), testis mRNA (anti-Mullerian hormone, amh; insulin-like growth factor-3, igf3) (relative mRNA expression); gonadosomatic index (GSI; %); plasma 11-ketotestosterone (11KT; ng/ml). (XLSX)