Underlying data for characterization of phycocyanobilin (PCB) biosynthesis in Galdieria sulphuraria

This dataset contains data for studies of phycocyanobilin (PCB) synthesis in the red alga Galdieria sulphuraria. Red algae such as G. sulphuraria utilize phycobilisomes for light harvesting. The phycobilisomes of early-branching organisms such as G. sulphuraria or Cyanidioschyzon merolae contain PCB chromophores but not phycoerythrobilin (PEB), in contrast to the phycobilisomes of other red algae. The studies reported in this dataset examine biosynthesis of PCB in G. sulphuraria and C. merolae, starting from biliverdin IX-alpha (BV), the last known common precursor for PCB and PEB. In cyanobacteria, phages, green algae, and land plants, conversion of BV into PCB or PEB is carried out by a family of enzymes called ferredoxin-dependent bilin reductases (FDBRs). The current studies demonstrate that G. sulphuraria, but not C. merolae, require the action of an additional isomerase to synthesize PCB.Deposited data include a phylogenetic a..., , ## Title: Underlying data for characterization of phycocyanobilin (PCB) biosynthesis in Galdieria sulphuraria

## Description of the Data and file structure

Data are deposited as a gzipped tarball containing a series of tab-delimited text files with unix newlines. After extraction, deposited data are in four folders:
I. FDBR_phylogeny
II. CmPCYA_in_vitro
III. GsPEBA_GsPEBB_in_vitro
IV. Galdieria_PEB_PCB_isomerase

I. **FDBR_phylogeny**:
A single phylogeny was inferred for this study, using PHYML version 3.1. The deposited files in this folder are all from this analysis.

Three files are deposited: the original multiple sequence alignment in CLUSTAL format, the input file for PHYML in PHYLIP format after removal of gap-enriched columns, and the final tree with transfer bootstrap expectation values in Newick format. The files are:
re107.aln (multiple sequence alingment in CLUSTAL format)
re107.05.phy (input file in PHYLIP format)
re107.05.phy_phyml_tree.txt (output file in Newick format)...,