Transcriptome analysis of heart tissue in wild type and cTnI R193H mutation mice based on RNA-seq

Aim:To explore the pathogenesis of restrictive cardiomyopathy (RCM) with cTnI mutation by using RNA-Seq to detect differentially expressed genes in WT and cardiac troponin I (cTnI) mutation mouse heart. Methods: The heart of WT mice and cTnI 193H mutant mice were extracted, all mRNA of myocardial tissue was extracted, and then transcribed into cDNA. After PCR amplification, the library was constructed, and high-throughput sequencing was performed. The sequencing results were analyzed to find the differentially expressed gene, and bioinformatics method was used to analyze the biological processes among those differentially expressed genes might be involved in. Results:A total of 52 differentially expressed genes were identified including 28 highly expressed and 24 low expressed ones ,of which 42 mRNA genes were differentially expressed (P<0.05). A total of 16 KEGG pathways were involved, and mainly participated in the immunological and metabolic processes. Conclusions:cTnI mutation may cause gene differential expression. Immune response,mitochondrial energy metabolism and myocardial cell differentiation may be involved in the progress of restrictive cardiomyopathy.The results might have offered new ideas for the research of RCM pathogenic mechanism. Examination of differentially expressed genes different in heart of WT and cTnI mutant mice, three biological repeats.