Expression of Human Lung Biopsies Before and at 6 hours of EVLP

Ex vivo lung perfusion restores normothermia, ventilation and circulation to donor lungs, typically after a period of cold ischemia. This allows donor lungs to be evaluated prior to transplantation. We used microarrays to study the biological response of human lungs to Ex Vivo Lung Perfusion. These are a subset of samples previously described in the paper: Yeung, Jonathan C., et al. Towards donor lung recovery—gene expression changes during ex vivo lung perfusion of human lungs. American Journal of Transplantation 18.6 (2018): 1518-1526. Samples were collected at the end of cold ischemia (CIT). Lungs were then placed on the EVLP circuit and a second sample was collected at 6 hours of EVLP.