Comprehensive identification and characterization of the transcripts, their expression levels and sub-cellular localizations

Comprehensive identification and characterization of the transcrtipts, their expression levels and sub-cellular localizations in mammalian genes were attempted. We used RNA-Seq method, in which next gene sequencing technology and shotgun sequencing of the RNAs were combined. We prepared RNA Seq libraries from several sub-cellular fractions and carried out the massive sequencing.