Radiofrequency driving antitumor effect of graphene oxide-based nanocomposites: a Hill model analysis - supplementary information

<b>Aim: </b>This report proposes using the Hill model to assess the benchmark dose, the 50% lethal dose, the cooperativity and the dissociation constant while analyzing cell viability data using nanomaterials to evaluate the antitumor potential while combined with radiofrequency therapy. <b>Materials &amp; methods: </b>A nanocomposite was synthesized (graphene oxide–polyethyleneimine–gold) and the viability was evaluated using two tumor cell lines, namely LLC-WRC-256 and B16-F10. <b>Results: </b>Our findings demonstrated that while the nanocomposite is biocompatible against the LLC-WRC-256 and B16-F10 cancer cell lines in the absence of radiofrequency, the application of radiofrequency enhances the cell toxicity by orders of magnitude. <b>Conclusion: </b>This result points to prospective studies with the tested cell lines using tumor animal models.