Supplemental Materials: Identification of proteins associated with the early restoration of insulin sensitivity after biliopancreatic diversion

ABSTRACT

CONTEXT: Insulin resistance (IR) is a risk factor for type 2 diabetes, diabetic kidney disease, cardiovascular disease and non-alcoholic steatohepatitis. Biliopancreatic diversion (BPD) is the most effective form of bariatric surgery for improving insulin sensitivity.

OBJECTIVE: To identify plasma proteins correlating with the early restoration of insulin sensitivity after BPD.

DESIGN: Prospective single centre study including twenty insulin resistant men with morbid obesity scheduled for BPD. Patient characteristics and blood samples were repeatedly collected from baseline up to four weeks post-surgery. IR was assessed by HOMA-IR, Matsuda Index and by studying metabolic profiles during meal tolerance tests. Unbiased proteomic analysis was performed to identify plasma proteins altered by BPD. Detailed plasma profiles were made on a selected set of proteins by targeted multiple reaction monitoring mass spectrometry (MRM/MS). Changes in plasma proteome were evaluated in ...