Statistical analysis of effects of glatiramer acetate (GA) on thrombin-induced CD62P upregulation and platelet aggregate formation for human and mouse platelets1.

1Human or mouse platelets were stimulated with thrombin in the presence of GA vs. Control (PBS) as described in Methods.2Optimal concentration of GA is 100 µg/ml for human platelets.3Optimal concentration of GA is 50 µg/ml for mouse platelets.4Mean fluorescence intensity (MFI) of CD62P expression on the surface of CD42a+CD61+ gated human or CD41+CD61+ gated mouse platelets was analyzed by FACS as described in Methods. Mean ± S.E. of five separate experiments is shown.5***, P6*, P7Percentages (%) of FCShiSSChi aggregated CD42a+CD61+ gated human or CD41+CD61+ mouse platelets was analyzed by FACS as described in Methods. Mean ± S.E. of five separate experiments is shown.8**, P