Oryza sativa Transcriptome or Gene expression. Oryza sativa

The aim of this experiment was to study the changes in the transcriptome of the IAC1131 rice genotype when exposed to a suite of multiple abiotic stress, either with or without pre-treatment with the plant hormone ABA (Abscisic acid). This is a follow-on from chapter 5, which uses the same experimental design, but this chapter is focused on analysis at the RNA transcriptome level. In this case we are looking at genes rather than proteins, with the aim of identifying stress response genes that are correlated with ABA signalling in both stressful and non-stressful conditions and examining ABA dependent and independent stress response genes in rice plants. Four groups of IAC1131 rice plants were grown including control plants incubated with ABA, control plants non-ABA incubated, stressed plants incubated with ABA and stressed plants non-ABA incubated, with leaf samples harvested after 0 days (control) and 4 days (stressed). RNA extraction was performed using a RNeasy Plant Mini Kit, and Illumina deep sequencing technology was used for total RNA Sequencing of samples.