Biofilm-Related, Time-Series Transcriptome and Genome Sequencing in Xylanase-Producing Aspergillus niger SJ1

In
this study, we found that biofilm formation is a critical factor
affecting the activity of Aspergillus niger SJ1 xylanase. Xylanase activity increased 8.8% from 1046.88 to 1147.74
U/mL during A. niger SJ1 immobilized
fermentation with biofilm formation. Therefore, we carried out the
work of genomic analysis and biofilm-related time-series transcriptome
analysis of A. niger SJ1 for better
understanding of the ability of A. niger SJ to produce xylanase and biofilm formation. Genome annotation
results revealed a complete biofilm polysaccharide component synthesis
pathway in A. niger SJ1 and five proteins
regarding xylanase synthesis. In addition, results of transcriptome
analysis revealed that the genes involved in the synthesis of cell
wall polysaccharides and amino acid anabolism were highly expressed
in the biofilm. Furthermore, the expression levels of major genes
in the gluconeogenesis pathway and mitogen-activated protein kinase
pathway were examined.