Core determinant of the in vitro enzyme activity of tenecteplase: primary structure over glycosylation modifications

This dataset supports the research presented in the manuscript titled "Core determinant of the in vitro enzyme activity of tenecteplase: primary structure over glycosylation modifications". The study aimed to establish a standardized in vitro enzymatic activity assay for tenecteplase (TNK) using an ACL TOP 700 automatic coagulation analyzer, and to elucidate whether primary structure or glycosylation modifications are the dominant determinant of TNK in vitro clot lysis activity. The dataset contains raw data from the following experiments: 1. Methodological validation of the automated clot lysis assay (specificity, accuracy, precision, linearity, repeatability and robustness) 2. Comparative clot lysis time determination of alteplase, tenecteplase and reteplase at equimolar concentrations 3. Consistency analysis between the automated coagulation method and the traditional bubble-rising method 4. Amino acid sequence verification of two commercial TNK products (TNK A and TNK B) via LC-MS/MS 5. Glycosylation profiling of TNK A and TNK B using HILIC-UPLC-FLD-MS 6. Clot lysis activity comparison of TNK products with distinct glycosylation modifications in buffers with and without human serum albumin (HSA) 7. Enzymatic activity changes during plasminogen-induced single-chain to two-chain conversion of TNK 8. Thermal denaturation temperature (Tm) determination of TNK under different conditions (control, DTT-treated, HSA+DTT-treated) using differential scanning calorimetry (DSC) All raw experimental data and GraphPad Prism project files corresponding to all figures in the manuscript are included in this dataset.