Chronic exposure of mouse primary PAC to nicotine decreases the levels of THTR-1 and THTR-2 proteins (A and B), mRNA (C and D) and hnRNA (E and F).

Western blotting was performed on whole cell proteins (60 μg) isolated from chronic nicotine-exposed mice and their controls. Real-time PCR was performed using mice THTR-1 and THTR-2 gene-specific primers. Data are mean ± SE from separate sets of samples from multiple mice and were normalized relative to ARPO and calculated by the relative relationship. *P P 0.05.