16S rRNA amplicon study of the Arabis alpina microbiota Raw sequence reads

We examined in long-term time-course experiments the bacterial root microbiota of the arctic-alpine perennial Arabis alpina in natural and controlled environments by 16S rRNA gene profiling. This allowed us to dissect the relevance of compartment, soil type, environment and host genotype on community assembly. Importantly, our experimental setup included non-flowering wild-type A. alpina plants and flowering pep1 mutant plants, allowing us to separate the contributions of soil residence time from flowering time.