Examining the thermotropic properties of large, circularized nanodiscs

Nanodiscs, soluble membrane mimetics composed of an amphipathic membrane scaffold protein encircling a lipid bilayer, are widely used in biophysical and structural studies of membrane proteins. Because many membrane proteins are responsive to their membrane environment, through specific protein–lipid interactions and bulk membrane shape and structure, it is important to understand the properties of lipid bilayers contained within nanodiscs in order to interpret studies using this technology. Nanodiscs are known to alter lipid properties, such as membrane thickness and melting temperature, and interactions with the nanodisc rim have been hypothesized to produce local perturbations in lipid structure and dynamics. Larger nanodiscs should compensate for this effect with a larger unperturbed area. To test this hypothesis, we examined the lipid bilayer properties of several lipids (DMPC, DPPC, POPC, DSPC) and soy polar lipid in circularized nanodiscs of 11 nm to 50 nm diameter using the envi..., , # Data from: Examining the thermotropic properties of large, circularized nanodiscs

Dataset DOI: [10.5061/dryad.cvdncjtfg](10.5061/dryad.cvdncjtfg)

## Description of the data and file structure

This repository contains raw fluorescence spectrum data for all samples included in the study associated with this repository as well as an archived version of the Python-based script used for data analysis of the raw fluorescence spectra and plotting scripts used in the study. The raw data were acquired on a Fluoromax+ spectrophotometer. The output was directly copied from the spectrophotometer software into CSV tables (raw data presented here). The data analysis script was run using Python 3.6.8.

Shortly, to assess how the incorporation of different lipid types into large, circularized nanodiscs was affected by nanodisc size, we measured the emission spectrum of different lipid types (asolectin, DMPC, DPPC, DSPC, and POPC) in nanodiscs ranging from 11 nm to 50 nm. Laurdan, a lipophilic, env...,