HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity. HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity

Purpose: Define HDAC4 signature Outcome: HDAC4 is required for the repression of a senescence signature. HDAC4 KO promotes the expression of inflammatory genes, the derepression of ERVs and the accumulation of DNA damage. All together these signalling pathways lead to permanent cell-cycle arrest in low grade tumor cells and pre-transformation models, while they weaken the replicative potential of primary normal cells. Overall design: 6 clones of SK-LMS-1 cells were created. Two controls: one expressing Cas9 as control, the other expressing Cas9 + sgRNA1. 4 HDAC4 KO clones were generated: 76 and 1231 (achieved through sgRNA1); 205 and 1254 (achieved through sgRNA2). Two biological replicates per clone were analyzed.