Metagenome of a microbial consortium obtained from the Tuna oil field in the Gippsland Basin, Australia.

In 2011, fluid samples were collected from the A7A oil well in the Tuna oil field (38°10' S, 148°25' E) in the Gippsland Basin, Australia. Chemical analyses of the water revealed the sample was pH 7.2, had a salinity of 2.7%, and contained 270 mg liter–1 calcium, 320 mg liter–1 magnesium, 1,200 mg liter–1 sulfate, and 1,170 mg liter–1 bicarbonate. The temperature in the reservoir was 102°C. One liter of formation water was filtered through a 0.2-?M polyvinylidene difluoride (PVDF) filter disc and subjected to genomic DNA extraction using a Meta-G-Nome DNA isolation kit (Epicenter Biotechnologies). The resultant DNA was sequenced using 100-bp paired-end Illumina HiSeq at the University of Western Sydney.