Spatial transcriptome analysis for squamous cell carcinoma arising from mature teratoma

Squamous cell carcinoma arising from mature teratoma (SCC-MT) is one of the rare ovarian malignancies. Therefore, the molecular background of SCC-MT remains largely unelucidated. In this study, we performed the spatial transcriptomics for SCC-MT. Archival fresh-frozen samples of SCC-MT were obtained from the National Cancer Center Biobank and Nagoya University. We prepared four slices from three patients with SCC-MT. Spatial transcriptomics was performed using the Visium Spatial Gene Expression Reagent Kits (10x Genomics) according to the manufacturer’s instructions.