Immunotherapy against amyloid beta is modulated by meningeal lymphatic function III

A growing body of evidence points to passive immunotherapy as a promising therapeutic strategy against AD. Herein, we show that meningeal lymphatic vasculature becomes dysfunctional in AD transgenic mice and that manipulating meningeal lymphatic drainage affects the outcome of anti-Aß immunotherapy. Overall design: Lymphatic endothelial cells (LECs) were isolated from the meninges of 20-24 months old male C57BL/6J mice, 4 weeks after intracisterna magna injection of 5 uL of AAV1-CMV-eGFP or AAV1-CMV-mVEGF-C (each at 1012 GC/µL). LECs were sorted by FACS directly into lysis buffer according to the following phenotype: Singlets DAPI-CD45-CD31+PDPN+. Each individual sample results from meningeal cells pooled from 10 mice.