Extracellular vesicle-based miR-122-5p correlates with liver fat and liver enzymes in metabolic dysfunction-associated steatotic liver disease.

This dataset contains FASTQ files, raw microRNA count data, and sample information from patients participating in the MULTISITE study, a longitudinal intervention study investigating the effects of a personalised weight-loss intervention on metabolic dysfunction-associated steatotic liver disease (MASLD).

Extracellular vesicles (EVs) were isolated from plasma using the Mag-Net method. RNA was extracted using the Norgen Biotek Exosomal RNA Extraction Kit and used to generate small RNA libraries with the NEXTFLEX® Small RNA-seq v4 Kit with UDIs. The protocol was modified to incorporate gel-based size selection instead of bead-based size selection, consistent with the approach used in the earlier NEXTFLEX® Small RNA-seq v3 Kit.

Libraries were quantified using the KAPA Library Quantification Kit and pooled in equimolar concentrations. The pooled library was sequenced on a NextSeq 500 using a High Output 75-cycle kit, generating single-index (8 bp) reads and 76 bp single-end reads.