Vanilla planifolia genome sequencing

Vanilla planifolia genomic DNA was sequenced in order to create an assembly suitable for read mapping and SNP calling. The selected V. planifolia accession is a clone of the same accession used for the previously published transcriptome (Rao 2014 A deep transcriptomic analysis of pod development in the vanilla orchid (Vanilla planifolia)). DNA was extracted using the CTAB method, submitted to the Duke GCB Sequencing Core for library preparation, and sequenced on two lanes of an Illumina HiSeq 4000 (paired-end 150 bp). Libraries with four insert sizes including mate-pairs (300 bp, 5-7 kb, 8-10 kb, and 10-12 kb) were used to create the draft genome.