Supplemental materials for Ko & Garrett (2025) mBio
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Table S1. Primers used in this studyFigure S1. Construction of an integration vector pDK017 for megL disruption. The EcoRI-SacI-digested pHS31 (pHS31-cut) was assembled with oriT fragment from pDN19 to generate pDK016. Then, the BamHI-NheI-digested pDK016 (pDK016-cut) was assembled with megL fragment carrying the in-frame stop codon (TAA) to generate pDK017.
创建时间:
2025-06-25



