Chromatin structure profiling of Zeb1-knockdown mouse retina endothelial cells by ATAC-seq

By comparing ATAC-seq from freshly isolated endothelial cells from control or Zeb1-knockdown retina, we observed an increase in the accessibility of chromatin structure in endothelial cells after Zeb1-knockdown. We used ATAC-seq to study the functional role of Zeb1 and identified genes that exhibited increase of accessibilities to their transcription start sites (TSS). Examination of chromatin accessibility using ATAC-seq in retina endothelial cells isolated from control or Zeb1-knockdown mice.