Whole genome mRNA expression profiling during Candida albicans growth in complete human serum

In order to get an insight into the complex interplay of mRNAs during the growth in complete human serum, the mRNA polulation of Candida albiacns NCCPF 400034 taken at 0, 20, 45, and 120 minutes was extracted and sequenced by Next Generation sequencing techniques. Overall design: Total RNA extraction from samples grown in YPD as control (02 samples) or complete human serum (06 samples: 02 replicates each time point ) and library preparared using Illumina kits. Sequenicng was performed using next generation sequencing platform.