RNA sequencing analysis of gene expression in the prefrontal cortex of mice treated with antidepressant drugs. RNA sequencing analysis of gene expression in the prefrontal cortex of mice treated with antidepressant drugs

We performed high-throughput profiling of gene expression in mouse prefrontal cortex in response to antidepressant treatment. BALB/c mice were treated with sertraline, duloxetine, or water for three weeks. Then we performed forced swim test to check their antidepressant-like behavior. We divided these animals to responder and non-responder based on their behavior in the forced swim test. We then conducted the transcriptomics analysis and found specific and distinct genes in responder and non-responder mice. Our study provided deep insights into the understanding of the molecular mechanisms underlying the behavioral response to antidepressants. Overall design: BALB/c mice were treated with sertraline, duloxetine, or water for three weeks. Total 5 groups were set up: mice received water (E), sertraline for responder (F) and non-responder (G), duloxetine for responser (H) and non-responder (I). Three replicates were used for each group.