Differential chromatine state and ER binding potentially induced by NR2F2 depletion.

ERa binding activity largely depends on access to binding sites on chromatin, which is facilitated in part by Pioneer Factors (PFs).We show that most binding events of NR2F2 occur together with the ERa binding sites.To explore whether NR2F2 may act as potential pioneer factor of ER, we performed a series of ChIP-seq genome wide in MCF-7. Since NR2F2 associates with chromatin prior to estrogen treatment and its depletion in MCF-7 cells did not affect ERa expression, we hypothesize NR2F2 may inhibit estrogen-dependent growth by modulating ERa recruitment. We performed ChIP-seq genome wide gainst ERa before and after NR2F2 depletion.Covalent modifications are a main chromatin property.To test whether NR2F2 favoured histone modification deposition on chromatin, we profiled ChIP-Seq of H3K4me1, H3K4me3, and H3K27ac following NR2F2 depletion in oestrogen-starved MCF-7 cells to gain comprehensive histone medication landscape. Overall design: Examination of 3 histone modifications and ER, FOXA1 binding in WT and NR2F2 KO MCF-7 cells.