RNA-Seq analysis of different tissue-types of developing rice seed

Aleurone layer differentiation completes by 9 DAF, and most of the nutrient filling occurs between 6-21 DAF, (Wu et al., 2016a, 2016b), so we decided to use 10 DAF time point for collection of pericarp, aleurone, embryo and endosperm tissues for RNA-Seq analysis. Since aleurone is mostly single layer, and it's almost impossible to separately collect pure pericarp and aleurone layers manually, so firstly we developed a method to collect different tissue types of developing grain through LCM and RNA extraction and Sequencing Overall design: 10 ng of total RNA was used for library preparation and sequencing using Illumina HiSeq2500 platform. 3 biological replicates for each of the tissue type (pericarp, aleurone, embryo, endosperm) were used. For each sample, we obtained in between of 17-32 million reads.