Alteration of Microglial Metabolism and Inflammatory Profile Contributes to Neurotoxicity in Frontotemporal Dementia 3

Transcriptome profiling of microglia cells carrying a G>C mutation at the last exon (exon six) of the CHMP2B gene linked to frontotemporal dementia (dbSNP: rs63750652) and of the corresponding isogenic controls. Heterozygous and homozygous mutant cells were obtained by precision CRISPR/Cas9 genome editing. All cells were derived from human induced pluripotent stem cells (healthy donor cell line). Overall design: Comparison between hiPSC-derived microglia carrying a G>C splice acceptor CHMP2B mutation linked to frontotemporal dementia, knocked-in via CRISPR/Cas9, and isogenic controls.