Supplementary Figure for the study `TNFpromotes Warburg effect in GCA`

Supplementary Figure for the study `TNFpromotes Warburg effect in GCA`

Supplemental Figure 1. Body weight at 10 weeks old and 20 weeks old in Control group, HFD+pH4BS group and HFD+pH4BS+CL group.
Supplemental Figure 2. Cross-sectional hematoxylin-eosin staining of the tumors in Gan mice.
(Upper) Representative cross-sectional images of the tumors in Control group-mice (Ctrl), pH4BS group-mice, HFD group-mice and HFD+pH4BS group-mice, (Lower) Tumor height of the Gan mice
Supplemental Figure 3. Representative photos of the F4/80 expression in tumor in immunohistochemical studies.
Immunostaining for a macrophage marker F4/80 (brown) in tumor in Gan mice,
(A. Control group, B. pH4BS group, C. HFD group, D. HFD+pH4BS group, E. HFD+pH4BS+CL group). F. The numbers of F4/80-positive cells (/HPF) in the tumor.
Supplemental Figure 4A. GSEA profile of Gan mice (HFD+pH4BS group vs. Control group)
4B. Enrichment plot of reactive oxygen species pathway and its downstream genes
Supplemental Figure 5. Predicted metabolic pathways in gut microbiome of pH4BS group (upper) and HFD group (lower) compared to Control group. The results of effect size (log fold change) and Bonferroni adjusted 95% confidence intervals using ANCOM-BC were shown. Positively and negatively changed pathways are indicated in pale red and pale blue bars, respectively.
Supplemental Figure 6. Representative images of the liver in Control group, HFD+pH4BS group and HFD+pH4BS+CL group. A. Hematoxylin-eosin staining, Iba-staining, B. Numbers of Infiltrating Iba-positive cells in the liver tissue, C. Western blot analysis of liver tissue for Iba1 and GAPDH.
Supplemental Figure 7. Difference in microbial phenotype between HFD+pH4BS group-mice and HFD+pH4BS+CL group-mice. A. Relative abundance of microbial taxa at class level. Stacked bar plots (left) and the relative abundance of Clostridia (right) are shown. B. Box plots of Firmicutes/Bacteroidetes (F/B) ratio.
Supplemental Figure 8. Representative images of western blot analysis (right) and lactic acid production (left) in MKN7 cells treated with E. coli LPS
Supplemental Figure 9. Mucosal Barrier damage of the ileum of Gan mice and Nrf2-KO Gan mice. A. Representative images of the hematoxylin-eosin staining and ZO-1 immunostaining of the ileal mucosa and the liver of the Gan mice treated with HFD+pH4BS (left) and the Nrf2-KO-Gan mice treated with HFD+pH4BS (right). B. Representative images of western blot analyses for the ZO-1 expression in the ileal mucosa and the LPS and TLR4 expressions in the liver.